Evaluation of Repeated Dose (Sub Chronic) Toxicity Study of Shilasindur Prepared by Two Different Methods Along with Its Characterization and Antimicrobial Activity

Introduction: Rasaushadhies (metal and mineral) preparations are unique that include Bhasma, herbo-mineral Kupipakwa rasayana hold a significant place in Ayurvedic pharmaceutics. Nearly 70 % of formulations combination one or more metallic/mineral with several herbs which have supporting role improving efficacy, relieving symptoms the disease to achieve long healthy life. Shilasindur is containing mercury, sulphur arsenic disulphide indicated skin disorders, respiratory tract disorders other infectious diseases.
 Aim Objectives: Evaluation repeated dose (Sub chronic) toxicity study along its characterization antimicrobial activity.
 Materials & Methods: will be prepared three batches as per classical reference by using Electric Muffle furnace. The formulation assessed Organoleptic characters, physicochemical parameters ICP – AES (Inductively coupled plasma atomic emission spectroscopy), FTIR (Fourier-transform infrared XRD (X-Ray Diffraction). Sub chronic carried out according OECD guidelines 408 452 well CCRAS safety toxicity. Epsilometer test (E-test) used check activity Shilasindur.
 Results: analytical establish pharmaceutical standardization. Toxicity done doses ensure safety. Statistical analysis applying unpaired t-test ANOVA test.
 Conclusion: If positive results obtained this work then it valuable contribution evidence drug safe for consumption at treatment doses.